Journal: Stem Cell Research & Therapy

Article Title: In vitro generation of Sertoli-like and haploid spermatid-like cells from human umbilical cord perivascular cells

doi: 10.1186/s13287-017-0491-8

Figure Lengend Snippet: Schematic of the optimized step-wise 5-week differentiation protocol that we developed for HUCPVC differentiation towards the Sertoli and germ cell lineages. DMEM Dulbecco’s modified Eagle’s medium, FBS fetal bovine serum, FSH follicle-stimulating hormone, GDNF glial cell-derived neurotrophic factor, HG high glucose, KOSR knockout serum replacement, LIF leukemia inhibitory factor, RA retinoic acid

Article Snippet: The small round cells were grown in S4-S5 media consisting of knockout DMEM (Gibco, USA) supplemented with 20% knockout serum replacement (Gibco, USA), 1× MEM vitamin solution (Life Technologies, USA), 1× MEM nonessential amino acids (Life Technologies, USA), 2 mM l -glutamine (Gibco, USA), 0.1 mM β-mercaptoethanol (Sigma, USA), 100 mg/ml penicillin/streptomycin (Gibco, USA), 1000 IU/ml follicle-stimulating hormone (FSH; EMD Serono, USA), 40 ng/ml GDNF (Sigma-Aldrich, Canada), 2 mM retinoic acid (Sigma-Aldrich, Canada), 1 mM testosterone (Sigma-Aldrich, Canada), and 0.5 ng/ml human insulin-like growth factor 1 (IGF-1; Sigma-Aldrich, Canada).

Techniques: Modification, Derivative Assay, Knock-Out